Regulation of Proliferation and Production of Prostate Specific Antigen by IL-1 in prostatic cancer cells line LNCaP

Y. Bouraoui Mechergui, C. Mezigh, N. Ben Rais, Z. Culig, R. Oueslati

Pro-inflammatory cytokines are produced by immune as well as non-immune cell types and mediate interactions between various cells. IL-6, TNFa and IL-1a are associated with tumour development and progression in human such as prostate cancer. Progression of prostate cancer to androgen independent state is commonly associated with increase in serum Prostate Specific Antigen (PSA), a useful biomarker for prostate cancer. The purpose of this study was to investigate the effect of proinflammatory IL-1 on LNCaP cells proliferation, PSA expression. The LNCaP ATCC cells were treated with IL-1 for 48 hours. The number of total cells and viable cells was counted with CASY cell counter + analyser system model TTC. PSA levels produced by LNCaP cells were measured by Advia Centaura, Bayer auto analyser. In Western Blot analysis, NF?B and AKT were revealed by the Odyssey antibody and detected by the scanner LI-COR Odyssey. The treatment of LNCaP cells with IL-1 inhibited cell proliferation and decreased PSA production. Western Blot analysis showed that AKT pathway was not affected by IL-1; whereas the NF?B activity was stimulated by this cytokine. PSA and LNCaP cells proliferation were down regulated by IL-1 through its up-regulation of the transcription factor NF?B. These finding, suggesting a cross talk between NF?B, PSA and others signalling pathways.
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